Journal: Nucleic Acids Research
Article Title: Functional and structural analysis of AT-specific minor groove binders that disrupt DNA–protein interactions and cause disintegration of the Trypanosoma brucei kinetoplast
doi: 10.1093/nar/gkx521
Figure Lengend Snippet: ( A ) SPR sensorgrams of HMGA1a (top left panel) and HMGB1 (top right panel) binding to dsDNA biotin-GGGGAATAATCGCGATTATTCCCCAATAATCGCGATTATT in HEPES 1 at 25°C. For HMGA1a, the best sensorgrams were obtained in HEPES 1 buffer, instead of MES or Phosphate buffer . ( B ) Binding curves for interaction of HMGA1a (lower left panel) and HMGB1 (lower right panel) with target DNA and fitting curve for a two-site affinity model.
Article Snippet: SPR binding and competitive experiments were performed at 25°C with a Biacore X-100 apparatus (Biacore GE).
Techniques: Binding Assay